Prime’s diagnostic assay uses extraction-less reverse transcription-loop-mediated isothermal amplification (RT-LAMP) which is massively scalable, can be fully automated for laboratories, and can produce results in <45 minutes.
RT-LAMP Technology Features
Sensitivity - High accuracy (>99% sensitivity and specificity)
Extractionless - Eliminates need for RNA extraction and built-in chemical viral inactivation
Rapid - Produces results in under 30 minutes compared to 120 minutes for PCR.
Multiplex - Can multiplex and test multiple microbes including positive and negative controls in a single reaction
Supply Chain - Uses US ISO certified contract manufacturers with multiple suppliers for our reagents.
Prime’s mNGS assays can identify known and novel mutations with single nucleotide accuracy supporting rapid turnaround times and small batch sizes up to 25 samples.
mNGS Technology Features
Rapid - Uses a rapid sample preparation protocol. Run on Illumina sequencers with rapid flow cells reducing sequencing time to <7 hours. Can process millions of reads in under 3 minutes which can take competitors several hours.
Full Automation - Custom APIs can upload to AWS cloud and automated pipelines will generate output report files.
Strain Level Sensitivity - Custom microbe identification methods can differentiate to strain level for diagnostic applications.
Denovo Gene Assembly - Can identify novel microbes and perform genome config creation and assembly for novel genomes.
Non-Targeted ID - Customized to target microbial genomes (lower cost and higher throughput) or run non-targeted identification for unknown pathogens.
Pooling Samples - Custom multiplexing methods and barcoding can pool hundreds of samples per sequencing run. Enables high volume sequencing with automated liquid handlers.
Prime’s PCR technology is massively scalable, can be fully automated for laboratories and produces results in <60 minutes while working with most extraction protocols.
Prime's Rapid PCR products:
Rapid PCR Technology Features
Workflow - Amplification of genetic material is rapid with our custom genotyping reagents and is performed in under 60 minutes.
High Specificity - Target mutations with high analytical sensitivity and specificity.
High Quality Detection - Genotyping assays that detect mutation and wildtype alleles, strong fluorescence enables accurate detection.
Device Compatability - Supports multiple qPCR instruments and fluorescence readers
Time - Simplified workflow that can go from sample to result in under 60 minutes